Valorization of crude glycerol and eggshell biowaste as media components for hydrogen production: A scale-up study using co-culture system

The properties of eggshells (EGS) as neutralizing and immobilizing agent were investigated for hydrogen (H2) production using crude glycerol (CG) by co-culture system. Eggshells of different sizes and concentrations were used during batch and repeated-batch fermentation. For batch and repeated-batch fermentation, the maximum H2 production (36.53±0.53 and 41.16±0.95mmol/L, respectively) was obtained with the EGS size of 33?m<x5 <75?m. Hydrogen production increased with the decreasing size of EGS. Eggshells maintained the fermentation pH (6.00?6.30) and provided immobilization support as confirmed by scanning electron microscopy. As media components, the EGS concentration of 0.25% (w/v) was found to be optimum for maximum H2 production (31.66±0.55mmol/L) and the production profile was comparable to H2 production (32.07±0.92mmol/L) obtained with all media components. In scale-up study with semi-continuous bioreactor (7.5L), almost 1.5-fold increase (in comparison to mono-culture) i.e. 312.12mmol-H2/L-of medium with 86.65% glycerol utilization was obtained.

» Author: Vinayak Laxman Pachapur, Ratul Kumar Das, Satinder Kaur Brar, Yann Le Bihan, Gerardo Buelna

» Reference: Bioresource Technology, Volume 225

» Publication Date: 01/02/2017

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This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement N° 690103