URBANREC

In this work, the industrial Saccharomyces cerevisiae PE-2 strain, presenting innate capacity for xylitol accumulation, was engineered for xylitol production by overexpression of the endogenous GRE3 gene and expression of different xylose reductases from Pichia stipitis. The best-performing GRE3-overexpressing strain was capable to produce 148.5?g/L of xylitol from high xylose-containing media, with a 0.95?g/g yield, and maintained close to maximum theoretical yields (0.89?g/g) when tested in non-detoxified corn cob hydrolysates. Furthermore, a successful integrated strategy was developed for the production of xylitol from whole slurry corn cob in a presaccharification and simultaneous saccharification and fermentation process (15% solid loading and 36?FPU) reaching xylitol yield of 0.93?g/g and a productivity of 0.54?g/L·h. This novel approach results in an intensified valorization of lignocellulosic biomass for xylitol production in a fully integrated process and represents an advance towards a circular economy.

» Author: Sara L. Baptista, Joana T. Cunha, Aloia Romaní, Lucília Domingues

» Reference: 10.1016/j.biortech.2018.07.068

» Publication Date: 01/11/2018

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